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ORIGINAL ARTICLE
Year : 2014  |  Volume : 4  |  Issue : 1  |  Page : 35-37

Molecular technique for detection of Leishmania infantum isolates in Iran


1 Department of Molecular, Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran
2 Department of Immunolgy School of Allied Health, University of Medical Sciences, Tehran, Iran

Correspondence Address:
Alireza Dolatyar Dehkharghani
#48, Shahrokh Alley, Zartoshtian Ave, Hafez Street, Tehran
Iran
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DOI: 10.4103/2229-5070.129160

PMID: 24754025

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Introduction: Leishmania infantum is the causative agent of autochthonous cutaneous and visceral cases of leishmaniasis and transmitted by female sandflies. The dogs are considered the main reservoir hosts; however, there are the reports on Leishmania infection in other animals. In this study, occurrence types of L. infantum isolates have been analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Materials and Methods: In this experimental study, 77 samples were cultured and prepared for microscopic study and examined through PCR-RFLP. The samples were used for both deoxyribonucleic acid (DNA) and smear-slide preparations. The DNAs were amplified by PCR for the detection of Leishmania subgenus and PCR products were restricted with HaeIII for the species differentiation. Results: The visceral Leishmania parasites were genotyped as L. infantum. It was also determined sensitivity in PCR (100%) was higher than microscopic examination. Conclusion: PCR-RFLP technique appears to be most sensitive for the detection and differentiation of L. infantum. There exists a relationship between genetic heterogeneousness and clinical manifestation and geographical regions of this disease in human.


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