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SYMPOSIUM
Year : 2014  |  Volume : 4  |  Issue : 2  |  Page : 90-95

Laboratory methods of identification of Entamoeba histolytica and its differentiation from look-alike Entamoeba spp.


Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research Gorimedu, Puducherry, India

Correspondence Address:
Subhash Chandra Parija
Dean- Research, Senior Professor, Department of Microbiology, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry - 605 011
India
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DOI: 10.4103/2229-5070.138535

PMID: 25250228

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Entamoeba histolytica , the causative agent of intestinal and extraintestinal amebiasis, is a common parasitic cause of significant morbidity and mortality in the developing countries. Hence, early detection and differentiation of pathogenic E. histolytica from nonpathogenic/commensal Entamoeba spp (Entamoeba dispar/Entamoeba moshkovskii/Entamoeba bangladeshi) plays a crucial role in clinical management of patients with amebiasis. Most diagnostic tests currently available do not reliably differentiate between the species of Entamoeba and are less sensitive, cumbersome to perform. Molecular-based methods are highly sensitive, easy to perform and differentiates the pathogenic Entamoeba from nonpathogenic species, serving the criteria for an ideal diagnostic test for amebiasis. Recently, microarray technology has been found to be a promising tool for the diagnostic and epidemiological evaluation of amebiasis.


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