Tropical Parasitology

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 3  |  Issue : 1  |  Page : 44--52

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen


Hany Mohamed Adel Abou-Elhakam1, Ibraheem Rabia Bauomy2, Somaya Osman El Deeb1, Azza Mohamed El Amir1 
1 Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
2 Department of Parasitology, Theodore Bilharz Research Institute, Giza, Egypt

Correspondence Address:
Hany Mohamed Adel Abou-Elhakam
Villa 330, First Touristic Village, 6th of October City, Cairo
Egypt

Background: Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility. Materials and Methods: In this study, Fasciola gigantica paramyosin (Pmy) antigen was early detected in cattle sera using sandwich enzyme-linked immunosorbent assay (ELISA), to evaluate the Pmy antigen performance in diagnosis. This work was conducted on 135 cattle blood samples, which were classified according to parasitological investigation into, healthy control (30), fascioliasis (75), and other parasites (30) groups. Results: The sensitivity of Sandwich ELISA was 97.33%, and the specificity was 95%, in comparison with parasitological examination, which recorded 66.66% sensitivity and 100% specificity, respectively. Conclusions: It was clear that the native F. gigantica Pmy is considered as a powerful antigen in early immunodiagnosis of fascioliasis, using a highly sensitive and specific sandwich ELISA technique.


How to cite this article:
Abou-Elhakam HM, Bauomy IR, El Deeb SO, El Amir AM. Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen.Trop Parasitol 2013;3:44-52


How to cite this URL:
Abou-Elhakam HM, Bauomy IR, El Deeb SO, El Amir AM. Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen. Trop Parasitol [serial online] 2013 [cited 2019 Dec 13 ];3:44-52
Available from: http://www.tropicalparasitology.org/article.asp?issn=2229-5070;year=2013;volume=3;issue=1;spage=44;epage=52;aulast=Abou-Elhakam;type=0