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  Indian J Med Microbiol
 

Figure 1: PCR amplicons of HtrA from Wu. bancrofti genomic DNA: (a) 1-100 bp ladder; 2-6 genomic DNA; 7 negative control. Colony PCR with gene specific PCR showing amplicons (b) and ECoRI digestion of plasmids (c) showing the inserts (~900 bp). A-E, 2-3, 6-9 are positive clones (white colonies); 4-5 negative control (blue colonies); 1 kit control

Figure 1: PCR amplicons of HtrA from Wu. bancrofti genomic DNA: (a) 1-100 bp ladder; 2-6 genomic DNA; 7 negative control. Colony PCR with gene specific PCR showing amplicons (b) and ECoRI digestion of plasmids (c) showing the inserts (~900 bp). A-E, 2-3, 6-9 are positive clones (white colonies); 4-5 negative control (blue colonies); 1 kit control