Close
  Indian J Med Microbiol
 

Figure 3: The expression of low molecular weight promastigote proteins are downregulated during prolong culture. AG83 promastigotes were harvested after one (a), four (b), seven (c), and nine (d) months in culture after transformation from amastigotes. Proteins (100 μg) from harvested parasite stocks were analyzed by two-dimensional polyacrylamide gel electrophoresis followed by immunoblotting with virulent aniserum. Low molecular weight proteins are marked by arrows inside the box

Figure 3: The expression of low molecular weight promastigote proteins are downregulated during prolong culture. AG83 promastigotes were harvested after one (a), four (b), seven (c), and nine (d) months in culture after transformation from amastigotes. Proteins (100 μg) from harvested parasite stocks were analyzed by two-dimensional polyacrylamide gel electrophoresis followed by immunoblotting with virulent aniserum. Low molecular weight proteins are marked by arrows inside the box