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  Indian J Med Microbiol
 

Figure 4: Low molecular weight antigens were appeared in promastigotes during the transformation from amastigotes in vitro. Parasite proteins during in vitro transformation from promastigotes and amastigotes were collected at regular intervals such as 0 h (lanes 4 and 8), 48 h (lanes 3 and 7), 72 h (lanes 2 and 6), and 96 h (lanes 1 and 5) and extracts (~25 ƒÊg) were analyzed in 12.5% SDS-PAGE and immunoblotted using virulent (lanes 1-4) and avirulent (lanes 5-8) serum. The low molecular weight proteins are indicated by arrows

Figure 4: Low molecular weight antigens were appeared in promastigotes during the transformation from amastigotes <i>in vitro</i>. Parasite proteins during <i>in vitro</i> transformation from promastigotes and amastigotes were collected at regular intervals such as 0 h (lanes 4 and 8), 48 h (lanes 3 and 7), 72 h (lanes 2 and 6), and 96 h (lanes 1 and 5) and extracts (~25 ƒÊg) were analyzed in 12.5% SDS-PAGE and immunoblotted using virulent (lanes 1-4) and avirulent (lanes 5-8) serum. The low molecular weight proteins are indicated by arrows