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  Indian J Med Microbiol
 

Figure 8: Pathogenic clones and an axenic mixed pathogenic population of Leishmania donovani promastigotes show identical immunoreactive protein pattern. Upper panel: Extracts of axenic culture of virulent AG83 promastigotes (lanes, 1 and 4), virulent clone C6 (lanes, 2 and 5), and D6 (lanes 3 and 6) were analyzed by immunoblotting with 1:20 dilution of virulent antiserum (lanes, 1-3) and avirulent antiserum (lanes, 4-6). Lower panel: Extracts of virulent AG83 (a), clones C6 (b), and D6 (c) were analyzed by two-dimensional polyacrylamide gel electrophoresis followed by immunoblotting with virulent serum. Low molecular weight proteins with in the box were marked

Figure 8: Pathogenic clones and an axenic mixed pathogenic population of <i>Leishmania donovani</i> promastigotes show identical immunoreactive protein pattern. Upper panel: Extracts of axenic culture of virulent AG83 promastigotes (lanes, 1 and 4), virulent clone C6 (lanes, 2 and 5), and D6 (lanes 3 and 6) were analyzed by immunoblotting with 1:20 dilution of virulent antiserum (lanes, 1-3) and avirulent antiserum (lanes, 4-6). Lower panel: Extracts of virulent AG83 (a), clones C6 (b), and D6 (c) were analyzed by two-dimensional polyacrylamide gel electrophoresis followed by immunoblotting with virulent serum. Low molecular weight proteins with in the box were marked