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  Indian J Med Microbiol
 

Figure 4: Transmission electron micrographs of the tegument and gastrodermis of untreated Cotylophoron cotylophorum and those treated with aqueous extract of pods of Acacia concinna (0.5 mg/mL) after 8 h exposure. (a) Untreated fluke showing compact tubercles (tu) (arrows) and intact tegumental syncytium (ts) (Bar-3 μm) (b) aqueous extract of pods of Acacia concinna-treated showing detachment of tegumental layer (arrows) and Subtegument (st) exposed with few vacuoles (v) (Bar-3 μm). (c) Untreated fluke showing intact nucleus (n) in a gastrodermis cell. The cytoplasm (c) is intense and evenly distributed. Granular endoplasmic reticulum (ger) is also seen (Bar-0.5 μm). (d) aqueous extract of pods of Acacia concinna-treated showing swollen granular endoplasmic reticulum (ger) (arrow) and vacuoles (v) in the cytoplasm. Disruption of perinuclear membrane with spillage of chromatin material (arrowhead) (Bar-0.22 μm). (e) Untreated fluke showing mitochondria (m) (arrows) in gastrodermis cells (Bar-1 μm). (f) aqueous extract of pods of Acacia concinna-treated showing swollen and abnormally shaped mitochondria (arrows) (Bar-1 μm)

Figure 4: Transmission electron micrographs of the tegument and gastrodermis of untreated <i>Cotylophoron cotylophorum</i> and those treated with aqueous extract of pods of <i>Acacia concinna</i> (0.5 mg/mL) after 8 h exposure. (a) Untreated fluke showing compact tubercles (tu) (arrows) and intact tegumental syncytium (ts) (Bar-3 μm) (b) aqueous extract of pods of <i>Acacia concinna</i>-treated showing detachment of tegumental layer (arrows) and Subtegument (st) exposed with few vacuoles (v) (Bar-3 μm). (c) Untreated fluke showing intact nucleus (n) in a gastrodermis cell. The cytoplasm (c) is intense and evenly distributed. Granular endoplasmic reticulum (ger) is also seen (Bar-0.5 μm). (d) aqueous extract of pods of <i>Acacia concinna</i>-treated showing swollen granular endoplasmic reticulum (ger) (arrow) and vacuoles (v) in the cytoplasm. Disruption of perinuclear membrane with spillage of chromatin material (arrowhead) (Bar-0.22 μm). (e) Untreated fluke showing mitochondria (m) (arrows) in gastrodermis cells (Bar-1 μm). (f) aqueous extract of pods of <i>Acacia concinna</i>-treated showing swollen and abnormally shaped mitochondria (arrows) (Bar-1 μm)