Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 2280
Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Login 
Year : 2016  |  Volume : 6  |  Issue : 1  |  Page : 78-81

Seroprevalence of Entamoeba histolytica from a tertiary care hospital, South India

Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India

Correspondence Address:
Subhash Chandra Parija
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry - 605 006
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2229-5070.175116

Rights and Permissions

Introduction: Amebiasis is the third leading cause of death after malaria and schistosomiasis. Diagnosis is based on microscopy, culture, isoenzyme analysis, and serology-based techniques. In resource-limited nation such as India where polymerase chain reaction cannot be employed, serology is considered to be the reliable diagnostic tool. To find the seroprevalence of Entamoeba histolytica IgG antibody by enzyme-linked immunosorbent assay (ELISA) among the liver abscess cases and healthy controls. Materials and Methods: Commercially available RIDASCREEN Entamoeba IgG ELISA kit was used to evaluate the samples as per manufacturer's instruction. Results : A total of 322 samples were evaluated by ELISA. 94/157 (59.87%) were positive for amebic liver abscess cases, 2/13 (15.38%) were positive in suspected amebiasis group, 5/15 (33.3%) were positive in nonamoebic hepatic disorder group, 5/39 (12.8%) were positive in other parasitic disorders, and 2/98 (2.04%) were positive in presumed healthy controls. The sensitivity and specificity of the assay were found to be 56% and 92%, respectively. Conclusion: In an endemic nation such as India and other developing countries, ELISA can be used as a routine surveillance test in a clinical setup to detect amoebiasis if the cases are judicially evaluated along with the other routine tests.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded25    
    Comments [Add]    
    Cited by others 5    

Recommend this journal