Tropical Parasitology

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 2  |  Page : 124--129

A hospital-based study on the prevalence of trichomoniasis and evaluation of accuracy of various diagnostic techniques


Sweety Singh1, Rumpa Saha1, Amita Suneja2, Shukla Das1 
1 Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi University, Delhi, India
2 Department of Gynaecology and Obstetrics, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi University, Delhi, India

Correspondence Address:
Rumpa Saha
Department of Microbiology, 3rd Floor, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi University, Delhi - 110 095
India

Background: The sexually transmitted infections (STIs) caused by Trichomonas vaginalis have been associated with adverse pregnancy outcomes and increased risk of HIV transmission. Trichomoniasis remains underreported despite being easy to diagnose and treat. Moreover, availability of battery of diagnostic tools causes dilemma on the most appropriate techniques to be used. Aims and Objectives: The purpose of this study was to determine the prevalence of T. vaginalis and its diagnostic accuracy employing various diagnostic techniques in women presenting with vaginal discharge in gynecological outpatient department (GOPD) of our tertiary care hospital. Materials and Methods: Five vaginal swabs were collected from 204 patients with symptomatic vaginal discharge attending GOPD. Wet mount microscopy, Giemsa and acridine orange staining, culture in Kupferberg media and InPouch™ TV culture system, and polymerase chain reaction (PCR) were performed and compared. Results: The prevalence of T. vaginalis was 1.96% in the present study. Wet mount microscopy, staining method, and culture detected 1.96% of cases, whereas PCR detected 2.45% of cases. Conclusion: The prevalence of T. vaginalis was <3% among symptomatic vaginal discharge patients from GOPD. Although PCR had a higher detection rate, there was no significant difference in sensitivity and specificity between other diagnostic methods (direct wet mount, Giemsa/acridine orange staining, and InPouch™ TV culture system). Hence, the availability in a particular setting would determine the methods of choice to be used for the diagnosis of T. vaginalis.


How to cite this article:
Singh S, Saha R, Suneja A, Das S. A hospital-based study on the prevalence of trichomoniasis and evaluation of accuracy of various diagnostic techniques.Trop Parasitol 2020;10:124-129


How to cite this URL:
Singh S, Saha R, Suneja A, Das S. A hospital-based study on the prevalence of trichomoniasis and evaluation of accuracy of various diagnostic techniques. Trop Parasitol [serial online] 2020 [cited 2021 Dec 7 ];10:124-129
Available from: https://www.tropicalparasitology.org/article.asp?issn=2229-5070;year=2020;volume=10;issue=2;spage=124;epage=129;aulast=Singh;type=0